Table 1 Primers used in this study
Target gene | Sequence | Length (nt) | Note |
|---|---|---|---|
TNF-α-F | CCCCTCAGCAAACCACCAAG | 20 | |
TNF-α-R | CTTGGCAGATTGACCTCAGC | 20 | |
TRAF3-F | GAGCAAGGAGGCTACAAGGAG | 21 | [32] |
TRAF3-R | CATGCAGCTCTCGCAGAAC | 19 | |
Actin-F | TGGCACCCAGCACAATGAA | 19 | [32] |
Actin-R | CTAAGTCATAGTCCGCCTAGAAGCA | 25 | |
P- TRAF3-overexpress -F | ATGGAGTCAAGCAAAAAGATGGA | 23 | |
P- TRAF3-overexpress -R | GGGGTCAGGCAGATCCGA | 18 | |
miR-3473-TRAF3-F | CTAGTGGTTCTAGAAAGTGTCAGTTTAACCAGA TCTCTCTCCACCACCAGAACTTTGTCTCTGCCA | 60 | Sangon |
miR-3473-TRAF3-R | AGCTTGGCAGAGACAAAGTTCTGGTGGTGGAG AGAGATCTGGTTAAACTGACACTTTCTAGAACCA | ||
miR-3473-TRAF3-mut-F | CTAGTGGTTCTAGAAAGTGTCAGTTTAACCAGA TCTGTTTGCACCACCAGAACTTTGTCTCTGCCA | 60 | |
miR-3473-TRAF3-mut -R | AGCTTGGCAGAGACAAAGTTCTGGTGGTGCAA ACAGATCTGGTTAAACTGACACTTTCTAGAACCA |