From: Bacillus subtilis 5′-nucleotidases with various functions and substrate specificities
Substrate | K M (mM)a | V max (μmol min−1 mg protein−1)a | k cat (s−1)a |
---|---|---|---|
MIMP | 0.076 ± 0.006 | 0.82 ± 0.16 | 2.9 ± 0.33 × 103 |
IMP | 1.1 ± 0.083 | 0.078 ± 0.0084 | 1.8 ± 0.59 × 102 |
AMP | 1.8 ± 0.13 | 0.030 ± 0.00022 | 5.3 ± 0.039 × 101 |
GMP | 1.9 ± 0.026 | 0.169 ± 0.028 | 3.0 ± 0.34 × 102 |
CMP | 1.6 ± 0.017 | 0.049 ± 0.014 | 1.5 ± 0.52 × 102 |
UMP | 2.4 ± 0.052 | 0.11 ± 0.0099 | 9.8 ± 4.6 × 102 |
G6P | 2.6 ± 0.51 | 0.021 ± 0.010 | 3.7 ± 1.3 × 101 |
β-glycerophosphate | 0.49 ± 0.035 | 1.2 ± 0.32 | 5.7 ± 1.2 × 102 |