Fig. 3From: Bacillus subtilis 5′-nucleotidases with various functions and substrate specificitiesGrowth curves of B. subtilis strains. Strains 168 (a), NON01 (b ΔycsE), NON02 (c ΔyktC), NON03 (d ΔycsE ΔyktC), NON05 (e and f, ΔyqeG Pspac-yqeG), and NON06 (g and h, ΔycsE ΔyktC ΔyqeG Pspac-yqeG) were inoculated into liquid medium and their growth was monitored. At the times indicated by the arrowheads, diamide was added to final concentrations of 0 mM (open circle), 1 mM (solid square), and 4 mM (solid triangle). Strains NON05 and NON06 were grown in the presence (e and g) and absence (f and h) of 1 mM IPTG. All experiments were repeated more than three times and similar results were observedBack to article page