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Fig. 3 | BMC Microbiology

Fig. 3

From: In vivo functional and molecular characterization of the Penicillin-Binding Protein 4 (DacB) of Pseudomonas aeruginosa

Fig. 3

D,D-peptidase activities on peptidoglycan of Pseudomonas aeruginosa. HPLC chromatograms of peptidoglycan obtained from reference strain UCBPP-PA14 (PA14WT), PA14WT/pHERD-PBP4 induced with L(+)-arabinose 0.2 %, PA14WT treated with cefoxitin (FOX), and PA14WT/pHERD-PBP4 induced with L(+)-arabinose 0.2 % and treated with cefoxitin (FOX). The following muropeptides are identified: M3, monomer disaccharide tripeptide; M4, monomer disaccharide tetrapeptide; M5, monomer disaccharide pentapeptide; M3L, monomer disaccharide tripeptide associated with lipoprotein; D43, dimer disaccharide tetrapeptide-tripeptide; D44, dimer disaccharide tetrapeptide-tetrapeptide; D44N, anhydrous dimer disaccharide tetrapeptide-tetrapeptide; T444, trimer tetrapeptide-tetrapeptide-tetrapeptide; T444N, anhydrous trimer tetrapeptide-tetrapeptide-tetrapeptide. Arab., arabinose; A204, absorbance at 204 nm, arbitrary units

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BMC Microbiology

ISSN: 1471-2180

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