Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42

Table 8 Regression statistics of CCD for optimization of cholesterol oxidase production by Streptomyces cavourensis strain NEAE-42

Factor	Coefficient estimate	Standard error	95 % CI Low	95 % CI High
Intercept	20.365	0.358	19.569	21.162
X₁ (initial pH)	−0.929	0.237	−1.457	−0.400
X₂ (cholesterol)	−0.498	0.237	−1.026	0.031
X₃ (ammonium sulphate)	−1.547	0.237	−2.075	−1.018
X₁ X₂	0.063	0.310	−0.628	0.753
X₁ X₃	−0.240	0.310	−0.931	0.450
X₂ X₃	−1.132	0.310	−1.823	−0.442
X₁ ²	−2.281	0.231	−2.795	−1.766
X₂ ²	−1.170	0.231	−1.685	−0.656
X₃ ²	−4.170	0.231	−4.684	−3.655
Std. Dev.	0.8766	R-Squared		0.9794
Mean	15.1615	Adj R-Squared		0.9608
C.V. %	5.7815	Pred R-Squared		0.8434
PRESS	58.3337	Adeq Precision		23.2242
C.V: Coefficient of variation

ISSN: 1471-2180