Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42

Table 6 Regression statistics and analysis of variance (ANOVA) for the experimental results of Plackett-Burman design used for cholesterol oxidase production by Streptomyces cavourensis strain NEAE-42

Source	SS	MS	F-value	t -Stat	P-value Prob > F	Confidence Level (%)
Model	86.172	5.069	101.190	15.705	0.0098*	99.02
A	1.306	1.306	26.067	−1.724	0.0363*	96.37
B	3.476	3.476	69.398	2.814	0.0141*	98.59
C	19.244	19.244	384.161	−6.620	0.0026*	99.74
D	0.629	0.629	12.553	1.196	0.0713	92.87
E	12.849	12.849	256.492	5.410	0.0039*	99.61
F	3.568	3.568	71.219	−2.851	0.0138*	98.62
G	0.356	0.356	7.100	0.900	0.1167	88.33
H	12.386	12.386	247.254	5.311	0.0040*	99.6
J	1.970	1.970	39.336	−2.118	0.0245*	97.55
K	11.494	11.494	229.447	5.117	0.0043*	99.57
L	15.856	15.856	316.522	6.009	0.0031*	99.69
M	0.081	0.081	1.626	0.431	0.3304	66.96
N	0.844	0.844	16.847	−1.387	0.0545	94.55
O	0.400	0.400	7.992	0.955	0.1057	89.43
P	0.058	0.058	1.149	0.361	0.3959	60.41
Residual	0.100
Cor Total	86.272
Std. Dev.		0.2238		R-Squared		0.9988
Mean		2.3269		Adj R-Squared		0.9890
C.V. %		9.6187		Pred R-Squared		0.8839
PRESS		10.0187		Adeq Precision		32.5110

SS - sum of squares, MS- mean square, F: Fishers’s function, P: Level of significance, PRESS the predicted residual sum of squares, CV %-the coefficient of variation%
*Significant values

ISSN: 1471-2180