Fig. 10
From: Disruption of the carA gene in Pseudomonas syringae results in reduced fitness and alters motility
Disruption of carA enhances biofilm formation. Biofilm formation by P. syringae DC3000, ΔargR, ΔP32, and ΔcarA. Cells were grown for 72 h at 28 °C in 96-well microtiter plates containing nutrient broth, and surface-associated biofilm formation was analyzed by crystal violet staining of the adherent biofilm, extraction of the crystal violet with acetic acid, and measurement of the absorbance (OD570). All experiments were done in triplicate with at minimum of three technical repeats. Data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey HSD for pair-wise comparisons. Asterisks indicate statistically significant difference (P < 0.003 by ANOVA; P < 0.01 using Tukey HSD)