Fig. 2From: The MarR-like protein PchR (YvmB) regulates expression of genes involved in pulcherriminic acid biosynthesis and in the initiation of sporulation in Bacillus subtilis Involvement of YvmB as repressor in the control of yvmC-cypX expression. a The strains were cultivated in MS medium containing 100 μM FeCl3 for 16 h. at 37 °C in flasks with continuous agitation at 200 rpm. BSB1 wild-type strain; BSAS82 ΔyvmB::aphA3; BSAS147 ΔyvmB::aphA3 cypX::lacZ. Cultures were transferred in tubes for the pictures. b and c Expression of a cypX::lacZ transcriptional fusion was compared in wild-type and ΔyvmB cells. Strains were grown in LB medium. Growth was monitored by measuring OD600: grey curves. β-galactosidase activity of the cypX::lacZ fusion is indicated: black circles, in the wild-type (strain BFA815); red squares, in ΔyvmB cells (strain BSAS147). Values are the means from at least four independent experimentsBack to article page