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Table 3 Determination of diagnostic performance ability of blood culture, N-PCR and Q-PCR assays considering clinically diagnosed typhoid fever (CDTF) cases (n = 110) positive by any of the test methods as standarda

From: Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis

Tests

Sensitivity (%, 95 % CI)

Specificity (%, 95 % CI)

PPV (%, 95 % CI)

NPV (%, 95 % CI)

LR+(95 % CI)

LR-(95 % CI)

Efficiency (%)

Culture

24/81, 29.6 (20.0–40.8)

29/29, 100.0 (87.9–100.0)

24/24, 100.0 (85.6–100.0)

29/86, 33.7 (23.9–44.7)

29.6/0, ∞

70.4/100, 0.70 (0.61–0.81)

53/110, 48.2b, c

N-PCR

66/81, 81.5 (71.3–89.2)

29/29, 100.0 (87.9–100.0)

66/66, 100.0 (88.3–100.0)

28/44, 65.9 (49.0–79.0)

81.5/0, ∞

18.5/100, 0.19 (0.12–0.3)

95/110, 86.4b, d

Q-PCR

74/81, 91.4 (83.0–96.4)

29/29, 100.0 (84.4–100.0)

74/74, 100.0 (95.0–100.0)

29/36, 80.6 (64.0–91.8)

91.4/0, ∞

8.6/100, 0.09 (0.04–0.18)

103/110, 93.6c, d

  1. CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value; LR+, positive likelihood ratio; LR-, negative likelihood ratio
  2. aEighty one CDTF with any one test positive result as true positive and 29 laboratory-confirmed non-typhoid cases as negative controls
  3. b, c p < 0.0001 using McNemer test
  4. d p > 0.1 using McNemer test