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Fig. 8 | BMC Microbiology

Fig. 8

From: Deciphering the mode of action of a mutant Allium sativum Leaf Agglutinin (mASAL), a potent antifungal protein on Rhizoctonia solani

Fig. 8

Identification of potential interactors of mASAL. a Representative 2-DE profile of the total protein extracted from R. solani in the pH range of 4–7 stained with Coomassie Brilliant Blue R-250 (b) Ligand blot of the same gel challenged with mASAL with subsequent incubation with mASAL specific antibody and respective secondary antibody. Arrows and boxes indicate spots with similar positioning in both A and B that are selected for analysis using LC MS/MS. M represents molecular weight marker. c Western blots showing co-immunoprecipitation of R. solani ATPase (lane 2), HSP 70 (lane 4) and Actin (lane 6) with recombinant mASAL from the total cell lysates of the pathogen. Lanes 1, 3 and 5 show the specificity of the antibodies used for ATPase, HSP 70 and Actin recognition respectively in R. solani total cell lysates. In each of the three cases mentioned above, the antibodies recognized single targets observed as single bands at expected size

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BMC Microbiology

ISSN: 1471-2180

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