Fig. 2From: Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species Comparative testing of mAb pairs in an antigen capture ELISA. To evaluate optimal antibody combinations, each of the 11 mAbs was used as a capture or detection (biotin-conjugate) antibody at a concentration of 10 μg/mL. Optical densities were measured for each antibody combination using gamma-irradiated B. melitensis (16 M) cells at a concentration of 107 cells/mLBack to article page