Figure 5

SYTO9/PI staining analyzed with confocal microscopy. Fluorescence images of the same samples at 528 nm (green) for SYTO9 signal, 645 nm (red) for PI signal and merged images are shown. 50:50 ratio of live and dead cells of P. aeruginosa (left) and S. aureus (right) was used. The cells were stained with SYTO9 and PI. Bacteria exhibiting red or yellow fluorescence are considered as dead cells.