Figure 4

Analysis of Hog1 phosphorylation in response to CUR treatment in HOG pathway mutants. (A and B) Wild type and HOG pathway mutant yeast strains were grown in SC media until they reached the exponential phase. Cells were treated with 100 μM CUR for 1 h and protein was extracted as described in the Materials and Methods. The western blot membranes were probed for phospho-Hog1. As a loading control, the same blots were reprobed with anti-TBP antibody. Equal protein loading was further confirmed by Ponceau S staining of the western blot membrane.