Table 6 Real-time quantitative RT-PCR confirmation of selected genes
From: Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
Locus no.a | Primer sequence (5′-3′)b | Product size (bp) |
|---|---|---|
16S rRNA | F: TGTTACAATGGGAGGGACAAAGGG | 118 |
R: TTACTAGCGAATCCAGCTTCACGG | ||
PG0090 | F: CAGAAGTGAAGGAAGAGCACGAAC | 197 |
R: GTAGGCAGACAGCATCCAAACG | ||
PG0195 | F: TCCACGGCTGAGAACTTGCG | 149 |
R: TGCTCGGCTTCCACCTTTGC | ||
PG1545 | F: CCAAACCCTCAACCACAATC | 142 |
R: GGTACCGGCTGTGTTGAACT | ||
PG0593 | F: CGTGTGGGAGAGTGGGTATTGG | 175 |
R: CGCCGCTGTTGCCTGAATTG | ||
PG1089 | F: CCATCGCGATCGATGATCAGGTAA | 104 |
R: GGCATAGTTGCGTTCAAGGGTTTC | ||
PG1019 | F: TTCGCAGTATCCCATCCAAC | 126 |
R: TCCGGCTCATAGACTTCCAA | ||
PG1180 | F: CAGTCTGCCACAGTTCACCA | 124 |
R: CCCTACACGGACACTACCGA | ||
PG1983 | F: GCTCTGTGGTGTGGGCTATC | 146 |
R: GGATAACAGGCAAACCCGAT | ||
PG0885 | F: CAGATCCAAATCGGGACTGA | 156 |
R: GTAGAGCAAGCCATGCAAGC | ||
PG1181 | F: GATGAATTCGGGCGGATAAT | 184 |
R: CCTTGAAGTGCTCCAACGAC |