Figure 2

Isolated murine nasal epithelial cells as observed under 40X Olympus light microscope on different days post-seeding. A) and B) unstained and stained preparation of isolated single cells seen on the day of isolation C) unstained and D) stained preparation of cultured NEC on day 2 post seeding. Nucleus is clearly evident in all the cells E) and F) cells as seen on day 3 post seeding of different shapes and sizes and G) Polygonal shaped NEC as seen on day 5 with significant increase in size as well. These cells were harvested, counted and used for adherence and invasion studies.