Table 2 The DNA sequences of primers used in this study.
From: Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repair
Oligonucleotide | Sequence (5'-3')* | Application | Source |
|---|---|---|---|
KT1b | gggaattccatatgcctgaattgccggaagtggaaacg | Cloning | This study |
KT2b | cgcgctcgagtttctgacagttcgggcaata | Cloning | This study |
TD146 | gaagtggaaacgacgttgcgcg | Sequencing | This study |
TD147 | cgtgccgcgctgccccaaagtttc | Sequencing | This study |
TD160 | ctcataccaaagtatcgc | Sequencing | This study |
TD161 | ttcgccccaccgtcctgc | Sequencing | This study |
TD46 | gctgttggaaaaactggg | Sequencing | This study |
TD47 | gcatacagataatccgtgc | Sequencing | This study |
spcFOR | cccagtggacataagcctgt | G-tract control, PCR/sequencing | [22] |
spcREV | agccgaagtttccaaaaggt | G-tract control, PCR/sequencing | [22] |
N248 | ggcggcatgaccc8oxog gaggcccatc | DNA substrate Containing 8oxoG lesion | Eurogen |
T248 | gatgggcctcc gggtcatgccgcc | DNA substrate, complementary to N248 | Eurogen |
1393 | gatgggcctca gggtcatgccgcc | DNA substrate, complementary to N248 | Eurogen |
1394 | gatgggcctcg gggtcatgccgcc | DNA substrate, complementary to N248 | Eurogen |
1395 | gatgggcctct gggtcatgccgcc | DNA substrate, complementary to N248 | Eurogen |
H7 | aacaacaacaaatgccgtctgaaccaacatgccgtctgaaaacaacaacaac | Undamaged DNA substrate | This study |
H8 | gttgttgttgttttcagacggcatgttggttcagacggcatttgttgttgtt | Undamaged DNA substrate, complementary to H7 | This study |