Table 1 Bacterial strains used in this study and MICs for KSM

E. coli

   DH5α

F^-ϕ80Δ(lacZY-argF)U169 deoR recA1 endA1 phoA hsdR17 supE44 λ^- thi-1 gyrA96 relA1 Δ(lacZ)M15

[56]

NT

   BW25113

Δ(araD-araB)567 ΔlacZ4787 (::rrnB-3) λ ^- rph-1 Δ(rhaD-rhaB)568 hsdR514

[29]

NT

   MC4100

F- araD139 Δ(argF-lac)U169 rpsL150 relA1 deoC1 rbsR fthD5301 fruA25 λ^-

[57]

150

   ATM809

MC4100 Δ ksgA::cat

This work

2500

ATM809 transformed with pRAK297 [Plac::E. coli ksgA in pGEMT]

This work

200

ATM809 transformed with pRAK325 [Plac:: C. psittaci 6BC UUG-ksgA in pGEMT]

This work

1000

   ATM810

ATM809 att:: E.coli ksgA

This work

200

   ATM812

ATM809 att:: C.trachomatis GUG-ksgA

This work

800

   ATM811

ATM809 att:: C.trachomatis AUG-ksgA

This work

400

   ATM815

ATM809 att:: C.trachomatis AUG-ksgAE9

This work

2500

C. psittaci 6BC

strain 6BC

T. Hatch

1100

   BCK₁

Spontaneous Ksm^R variant of C. psittaci 6BC with A₇₉₄G mutation in the 16S rRNA gene

[13]

>>5000

C. trachomatis L2

biovar lymphogranuloma venereum L2/434/Bu

H. Caldwell

800

   L2 ksgAE9

C. trachomatis L2 ksgAE9

This work

3000