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Table 3 Sequences of primers and probes used

From: Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosain sputum of cystic fibrosis patients

Primer/Probe

5'-3' Sequenced

Amplicon size (bp)

Reference or source

PAO1 Sa

PAO1 Aa

ACC CGA ACG CAG GCT ATG-TET

CAG GTC GGA GCT GTC GTA CTC

92

TIB Molbiol

oprL Fa

oprL Ra

ATG GAA ATG CTG AAA TTC GGC

CTT CTT CAG CTC GAC GCG ACG

504

[13, 28]

oprL-LC-FAMb

TGC GAT CAC CAC CTT CTA CTT CGA GT-FAM

/

TIB Molbiol

oprL-LC-ROXb

ROX-CGA CAG CTC CGA CCT GAA G

/

TIB Molbiol

oprL TMc

FAM-AGAAGGTGGTGATCGCACGCAGA-BBQ

/

TIB Molbiol

  1. a Primers
  2. b HybProbes
  3. c TaqMan Probe.
  4. d TET, FAM and ROX are fluorescent labels. BBQ: BlackBerry quencher