PCR formata | Cyclerc | Primers | Probes | Annealing temperature (°C)d | Last positive dilution |
---|---|---|---|---|---|
1. PCR + AGEb | 1 | PAO1 S/PAO1 A | None | 55 | 6 |
2. PCR + FCE | 1 | PAO1 S/PAO1 A | None | 55 | 7 |
3. real-time PCR + SybrGreen | 2 | PAO1 S/PAO1 A | None | 55 | 7 |
4. real-time PCR + HybProbes | 2 | oprL F/oprL R | oprL-LC-ROX/oprL-LC-FAM | 57 | 8 |
5. real-time PCR + TaqMan probeb | 2 | PAO1 S/PAO1 A | oprL TM | 55 | 8 |
6. real-time PCR + TaqMan probe | 3 | Not specified | Not specified | 60 | 8 |