Population diversity and antibody selective pressure to Plasmodium falciparum MSP1 block2 locus in an African malaria-endemic setting

Noranate, Nitchakarn; Prugnolle, Franck; Jouin, Hélène; Tall, Adama; Marrama, Laurence; Sokhna, Cheikh; Ekala, Marie-Thérèse; Guillotte, Micheline; Bischoff, Emmanuel; Bouchier, Christiane; Patarapotikul, Jintana; Ohashi, Jun; Trape, Jean-François; Rogier, Christophe; Mercereau-Puijalon, Odile

doi:10.1186/1471-2180-9-219

Table 3 Ewens-Watterson (EW) tests for neutrality for Pfmsp 1 block2 alleles from Dielmo, Senegal.

From: Population diversity and antibody selective pressure to Plasmodium falciparum MSP1 block2 locus in an African malaria-endemic setting

Year	Sample size	Observed F	Expected F	p-values
1990	46	0.3535	0.626	0.0201
1991	49	0.3536	0.6302	0.021
1992	43	0.3618	0.6213	0.0317
1993	63	0.3923	0.6463	0.0584
1994	54	0.3957	0.6366	0.0662
1995	51	0.4048	0.633	0.08
1996	68	0.3387	0.651	0.0051
1997	46	0.3573	0.626	0.0253
1998	76	0.3695	0.6575	0.0303
1999	28	0.398	0.5894	0.099
All	524	0.3622	0.7429	0.0108
	Size polymorphism				Size and sequence polymorphism
Year	N	Observed F	Expected F	p-value	N	Observed F	Expected F	p-value
	K1 family
1990	18	0.1728	0.17	0.6612	8	0.1562	0.1562	1
1991	22	0.095	0.099	0.577	11	0.157	0.1542	0.8934
1992	20	0.195	0.1789	0.7793	14	0.0816	0.0816	1
1993	33	0.0964	0.1379	0.0186	20	0.065	0.0607	1
1994	29	0.1249	0.1294	0.551	15	0.0756	0.0756	1
1995	28	0.148	0.1422	0.6971	18	0.1111	0.1113	0.6803
1996	26	0.1775	0.1757	0.6323	18	0.0988	0.1113	0.267
1997	20	0.245	0.1551	0.9901	11	0.0909	0.0909	1
1998	37	0.122	0.1316	0.4808	21	0.1111	0.0962	0.936
1999	14	0.1939	0.2125	0.417	8	0.125	0.125	1
All	247	0.1044	0.0957	0.7197	144	0.0245	0.0214	0.9088
	MAD20 family + Hybrid alleles
1990	18	0.1358	0.1273	0.8024	9	0.1605	0.1683	0.6858
1991	13	0.2071	0.2505	0.2629	8	0.1562	0.1562	1
1992	13	0.1834	0.1698	0.8471	9	0.1111	0.1111	1
1993	18	0.1728	0.1995	0.3267	13	0.1243	0.1208	0.9238
1994	12	0.1667	0.1973	0.2356	9	0.1358	0.1358	1
1995	10	0.32	0.2831	0.9022	9	0.1605	0.1683	0.6858
1996	23	0.2098	0.1906	0.7541	12	0.0972	0.0972	1
1997	16	0.1797	0.1886	0.5808	11	0.1736	0.1885	0.5419
1998	18	0.2037	0.2369	0.3518	10	0.14	0.1455	0.7227
1999	4	0.25	0.25	1	NA	NA	NA	NA
All	145	0.1177	0.1201	0.5816	90	0.0365	0.0407	0.2691
	RO33 family
1990	NA	NA	NA	NA	10	0.66	0.4919	1
1991	NA	NA	NA	NA	13	0.7396	0.7035	0.6469
1992	NA	NA	NA	NA	10	0.68	0.6826	0.6047
1993	NA	NA	NA	NA	12	0.8472	0.6975	1
1994	NA	NA	NA	NA	13	0.3609	0.3976	0.3849
1995	NA	NA	NA	NA	12	0.7222	0.6975	0.6347
1996	NA	NA	NA	NA	18	NA	NA	NA
1997	NA	NA	NA	NA	9	0.358	0.4797	0.1481
1998	NA	NA	NA	NA	20	0.745	0.7331	0.5446
1999	NA	NA	NA	NA	7	0.5102	0.6509	0.2358
All	NA	NA	NA	NA	124	0.6403	0.4419	0.8793

Ewens-Watterson tests by individual year during the 1990-1999 decade and for all years of the decade grouped together (All). In the top Table, tests were based on family frequency distribution. Three families were considered: K1, MAD20/Hybrids and RO33. The second part shows the results of the Ewens-Watterson tests within each family with alleles identified by size polymorphism only or both size and sequence polymorphism. For the RO33 family no size polymorphism was observed.
F: Homozygosity. N: sample size. NA: Not Available.

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ISSN: 1471-2180

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