Substrate | Temperature [°C] | Km [mM] | kcat [s-1] | kcat/Km [s-1mM-1] |
---|---|---|---|---|
ONPG | 10 | 5.75 ± 0.34 | 52.4 ± 0.72 | 9.12 ± 0.71 |
20 | 4.86 ± 0.37 | 81.0 ± 1.03 | 16.67 ± 1,60 | |
30 | 3.46 ± 0.29 | 123.9 ± 1.21 | 35.81 ± 3.66 | |
40 | 3.15 ± 0.27 | 169.9 ± 1.44 | 53.92 ± 5.56 | |
50 | 2.62 ± 0.21 | 212.4 ± 1.67 | 81.07 ± 7.76 | |
55 | 5.11 ± 0.32 | 71.2 ± 0.98 | 13.93 ± 1.14 | |
lactose | 10 | 77.54 ± 1.77 | 1.76 ± 0.11 | 0.023 ± 0.002 |
20 | 67.82 ± 1.74 | 2.36 ± 0.14 | 0.035 ± 0.003 | |
30 | 52.67 ± 1.71 | 4.81 ± 0.22 | 0.091 ± 0.007 | |
40 | 44.31 ± 1.73 | 5.73 ± 0.21 | 0.129 ± 0.010 | |
50 | 39.73 ± 1.72 | 6.98 ± 0.23 | 0.176 ± 0.014 |