Figure 1From: Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideumthrough targeting of multiple Rho family GTPasesKinetics of Yop expression in D. discoideum. (A) Expression of yopE was induced by removal of tetracycline (-Tet). At indicated time points (in hours), total RNA of 107 cells was separated on 1.2% agarose/6.6% formaldehyde gels, transferred onto a nylon membrane, and probed with DIG-labeled yopE. (B) Expression of GFP-Yop fusion proteins. Expression was induced by removal of tetracycline (-Tet). At indicated time points (in hours), total cell protein from 5 × 105 vegetative cells was separated on 15%polyacrylamide/0.1% SDS gels and blotted onto nitrocellulose. Blots were probed with a GFP-specific antibody.Back to article page