A two-genome microarray for the rice pathogens Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola and its use in the discovery of a difference in their regulation of hrp genes

Table 4 Expression profiles of hrp and rax genes in Xoo and Xoc cultured in XOM2 vs. PSB by microarray and semi-quantitative RT-PCR analysis

Locus ID	Product	P-value^a	Log2 ratio^a	RT-PCR^b	Locus ID	Product	P-value^a	Log2 ratio^a	RT-PCR^b
XOO0095	hpa1	0	1.529		XOCORF2625	hpa1	0.000307	1.219
XOO1379	hrpG	1.5E-09	1.102	+	XOCORF3400	hrpG	0.025035	0.086	0
XOO0094	hrcC	2.9E-11	1.094	+	XOCORF2442	hpaF	0.011623	0.019
XOO0076	hrpE	1.1E-11	0.974	+	XOCORF2448	hrpE	0.010426	0.757	+
XOO1380	hrpX	0.000154	0.410	+	XOCORF3402	hrpX	0.0822	-0.443	0
XOO0085	hrcU	3.1E-06	0.313	+	XOCORF2466	hpa2	0.099664	-0.064
XOO0090	hrpB5	0.345847	0.076		XOCORF2451	hrcS	0.000253	0.003
XOO4533	hrpB	0.222464	0.063	+	XOCORF2289	hrpB	0.063541	0.073	0
XOO0087	hrpB2	0.411161	0.057		XOCORF2457	hrpB2	0.119757	0.013	0
XOO0075	hpaB	0.444516	0.041		XOCORF2447	hpaB	0.029266	0.194
XOO0066	hrpF	0.792649	-0.019	+	XOCORF2444	hrpF	0.077523	0.036	0
XOO0079	hpaA	0.199054	-0.070	+	XOCORF2458	hrpB3	0.056872	0.026
XOO0077	hrpD6	0.252583	-0.073	+	XOCORF2449	hrpD6	0.049163	-0.093
XOO0089	hrpB4	0.01751	-0.152		XOCORF2459	hrpB4	0.45174	-0.038
XOO0091	hrcN	4.1E-05	-0.196		XOCORF2462	hrpB7	0.039424	-0.237
XOO0083	hpaP	0.004091	-0.202		XOCORF2454	hpaP	0.061767	-0.008
XOO3396	raxQ	0.407272	-0.038		XOCORF3280	raxQ	0.045558	0.088	0
XOO3544	raxA	0.807527	-0.014	0	XOCORF1002	raxA	0.274492	0.057
XOO3535	raxR	0.418945	0.056		XOCORF0993	raxR	0.786683	0.015	0
XOO3397	raxP	0.000764	0.183	0	XOCORF3279	raxP	0.014682	-0.018	0
XOO3534	raxH	0.001411	-0.191		XOCORF0994	raxH	0.319727	-0.054
XOO0927	raxC	0.001717	-0.352	-	XOCORF3127	raxC	0.123998	-0.162	0
XOO3545	raxST	4.2E-05	-0.288	0
XOO3543	raxB	0.422388	-0.051	0

^aResults of microarray analysis, as described in text. A log2 ratio of 0.8 is equivalent to a 1.75 fold relative increase in expression.
^aResults of RT-PCR, as described in text. +, up-regulated in XOM2; -, down-regulated in XOM2; 0, no change in transcript abundance detected, blank, not tested.

ISSN: 1471-2180