Figure 1

Southern blot showing hybridization of the probe for cje0215 of sequenced strain RM 1221 to DNA from isolates used in this study. Pst I cut genomic DNA was hybridized and blotted as summarized in the Methods section. Sizes of the Hind III-cut λ DNA standard are shown at the left of the figure. Lanes with visible bands were scored as positive for the presence of DNA sequence homologous to the probe, while those without visible bands were scored negative. Isolates were in lane: 1, NC 13254; 2, NC 13255; 3, NC 13256; 4, NC13257; 5, NC 13258; 6, NC 13259; 7, NC 13260; 8, NC 13261; 9, NC 13262; 10, NC 13263; 11, no DNA; 12, NC 13265; 13, NC 13266; 14 RM 1221; 15, no DNA; 16, 99–7046; 17, 00–0949; 18, 00–1597; 19, 00–2533; 20, 00–2538; 21, 00–2575; 22, 00–2814; 23, 00–2818; 24, 00–3477; 25, 00–4221; 26, 00–6200; 27, 03–1120; 28, RM 1221; 29, NC 13264.