Figure 6

Faecal bacterial community analysis using DGGE. (A) DGGE analysis, in a denaturing gradient of 40–60% urea and formamide, of the PCR products from the amplification of faecal bacterial genomic DNA from three healthy individuals (A, B and C) and a UC patient in active (D) and remission (R) states of the disease, using primers for 16S ribosomal gene region V6-V8. (B) DGGE analysis, in a denaturing gradient of 30–60% urea and formamide, of the PCR products from the amplification of faecal bacterial genomic DNA from three healthy individuals (A, B and C) and a UC patient in active (D) and remission (R) states of the disease, using primers for 16S rDNA region V3. For both gels, numbered arrows point to bands subjected to re-amplification and sequencing, and correspond to the amplicon identities in Table 4 (V6-V8) and Table 5 (V3). The ladder on each gel is used for quality control purposes only.