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Figure 2 | BMC Microbiology

Figure 2

From: A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities

Figure 2

Determination of the optimum washing temperature for the short oligonucleotide probes. Discrimination of the perfect match probe (BLON135a), against either the 1 nt (BLON135b) (grey bars) or 2 nt (BLON135c) (white bars) mismatch probes. Labelled 16S ribosomal DNA from a pure culture of Bifidobacterium longum was hybridised at 58°C and washed between 55°C and 66°C. Perfect match probe intensity at each different temperature was then divided either by the 1 nt or 2 nt corresponding mismatch binding, and error bars represent the standard error.

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BMC Microbiology

ISSN: 1471-2180

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