Figure 2
Multiplex PCR for identifying Salmonella H1 and H2 gene alleles. (a)Multiplex PCR for identifying H1 antigen gene alleles: i, g,m, r, and z10. Lanes 2–7: H1-1 multiplex PCR for i and g,m antigens. Lanes 9–14: H1-2, multiplex PCR for antigens r and z10. Lanes 1, 8, and 15: 100 bp MW standard; lane 2: H1-1 multiplex PCR control; lane 3: S. enterica serovar Typhimurium [i]; lane 4: S. enterica serovar Kentucky [i]; lanes 5 and 6: S. enterica serovar Enteritidis [g,m]; lane 7: no DNA control; lane 9: H1-2 multiplex PCR control; lane 10: S. enterica serovar Hadar [z10]; lane 11: S. enterica serovar Mbandaka [z10]; lane12: S. enterica Heidelberg [r]; lane 13: S. enterica serovar Infantis [r]; and lane 14: no DNA control. The sizes of the PCR amplicons are: 508 bp for i, 309 bp for g,m, 169 bp for r, and 363 bp for z10. (b)Multiplex PCR for identifying H2 antigen complexes I: 1,2, 1,5, 1,6, 1,7 and II: e,n,x, e,n,z15 respectively. Lanes 1 and 10: 100 bp MW standard; lane 2: multiplex PCR control for H2 antigen complexes I: 1,2; 1,5; 1,6; 1,7 and II: e,n,x; e,n,z15; lane 3: S. enterica serovar Typhimurium [1, 2]; lane 4: S. enterica serovar Infantis [1, 5]; lane 5: S. enterica serovar Anatum [1, 6]; lane 6, S. enterica serovar Bredeney [1, 7]; lane 7: S. enterica serovar Hadar [e,n,x]; lane 8: S. enterica serovar Mbandaka [e,n,z15]; and lane 9: no DNA control. The sizes of the PCR amplicons are 294 bp for H2 antigen complex I: 1,2; 1,5; 1,6; 1,7 and 152 bp for H2 antigen complex II: e,n,x and e,n,z15.