Figure 1

1(a) – Alignment of L. brevis S-layer protein sequences. The mature forms of S-layer proteins were aligned by ClustalW and the alignments were divided into stretches of 10 amino acids, from which the percentage of identical amino acids and amino acids with conserved substitutions were calculated. The following colours are used to indicate the percentages of identical and similar amino acids in each calculated stretch: white, 0–20%, light gray, 21–40%, medium gray, 41–60%, dark gray, 61–80%. 1(b) &#8211 Hydrophobicity of mature SlpA. The hydrophobicity was calculated according to Kyte and Doolittle [34] with a window of seven amino acids. 1(c) &#8211 Predicted pI values of the N- and C-terminal regions of L. brevis S-layer proteins. The lengths of the N- and C-terminal regions as well as the full lengths of the mature forms of the proteins are indicated in brackets.