Differential expression of lipoprotein genes in Mycoplasma pneumoniae after contact with human lung epithelial cells, and under oxidative and acidic stress

Table 2 Changes in M. pneumoniae lipoprotein gene expression after contact with human A549 cells for 2 hours, or after incubation in 20 mM H₂O₂ or at pH 5.5 for 10 min

Lipoprotein family	Gene annotation (NCBI)	Change in expression (fold) after:
		Contact with A549 cells#	P value	20 mM H₂O₂ for 10 min†	P value	pH 5.5 for 10 min§	P value
1	MPN084	1.1	0.714	0.5	0.439	1	0.910
	MPN591	1	0.966	0.8	0.645	1.6	0.275
	MPN592	0.9	0.880	0.9	0.906	0.9	0.825
	MPN083	1	0.934	1.7	0.504	1.3	0.605
	MPN588	1.9	0.002**	0.6	0.475	0.8	0.504
	MPN582	1.2	0.586	0.4	0.375	0.6	0.222
2	MPN199	2.5	0.003**	0.6	0.443	0.6	0.220
	MPN408	0.8	0.469	0.4	0.105	0.2	0.001**
	MPN200	3.3	0.006**	0.4	0.195	0.4	0.037*
	MPN152	1.2	0.677	0.2	0.104	0.2	0.020*
3	MPN436	0.9	0.802	0.4	0.094	0.9	0.836
	MPN444	1.3	0.549	0.7	0.240	0.8	0.555
	MPN489	1.4	0.286	0.6	0.033*	0.7	0.328
4	MPN456	2.2	0.010*	0.8	0.681	0.5	0.082
5	MPN011	1.6	0.044*	0.4	0.299	0.6	0.316
	MPN012	1.5	0.119	0.3	0.122	0.6	0.257
	MPN411	1.9	0.032*	0.5	0.345	0.7	0.442
	MPN271	1.2	0.638	0.6	0.451	0.9	0.794
	MPN505	0.8	0.602	0.3	0.095	1.5	0.302
6	MPN647	1.2	0.603	0.4	0.130	2.3	0.081
	MPN646	0.9	0.846	0.5	0.097	1.8	0.177
	MPN645	1.4	0.360	0.5	0.624	1.3	0.398
	MPN644	1.1	0.876	0.6	0.358	0.9	0.753
	MPN643	1.5	0.208	0.6	0.503	1.1	0.831
	MPN642	1.1	0.895	0.6	0.323	0.7	0.457
	MPN641	1.7	0.208	0.5	0.063	0.6	0.264
	MPN640	1	0.980	0.6	0.431	0.9	0.658
	MPN639	1.1	0.766	0.3	0.007**	0.8	0.514

* P value < 0.05.
** P value < 0.01.
# Mean of three independent experiments, each containing three biological replicates.
† Mean of four independent experiments.
§ Mean of three independent experiments.

ISSN: 1471-2180