Table 1 Oligonucleotides used for qPCR
Family | Gene annotation (NCBI) | Forward primer | Reverse primer |
|---|---|---|---|
HKGa | MPN430 | GACGTATTGGACGCCTTGTT | GTCGTTAACGGCAACGATCT |
HKG | MPN665 | GGCAAGAACACCCCTATT | TAGGATCACCTTCAAGCG |
HKG | MPN003 | GGGGAAGTGATTGGTGAT | TTGGTACCGTGTTCCTTG |
1 | MPN084 | AACCCATTTCTTTGCATTGG | GTTTTGGGGATTGCTGATGT |
| Â | MPN591 | ACACAACTGTGGGAATGCAA | CCCTGCCTTAGGTTTTTGGT |
| Â | MPN592 | TGCTTGCTCTGCTACGCTAA | GCTCAATGGTGGTTGAGGTT |
| Â | MPN083 | ACCAACGGTTTTTGAACAGC | GCATCATTGGGGTTGGATAG |
| Â | MPN588 | AAAGCCTTGGTTTCGATTCA | TGCCAGGAAGACTTGTTGTG |
| Â | MPN582 | TGTGCGAACCGTTGATTTTA | CTAATTTGCTTGGTGCGACA |
2 | MPN199 | AGTTTCCGCTAGTTCGTTGC | GTTTTTGCGGCATCTTCAAT |
| Â | MPN408 | ATTCCCATTTCCCTTTCCAC | CATTTGAGCACCGTTTTCCT |
| Â | MPN200 | TTCCGGTCTCTGTTTCGACT | TCTTTTTGTGCGCCCTTACT |
| Â | MPN152 | CGATTAATGGACCCGTTTTG | TCTTTGCACCGAAGTGACAG |
3 | MPN436 | CCCAGTCAAGGGTTAGGTCA | TCTTCGGCAAAGAAAGGAAA |
| Â | MPN444 | AACCGAAGTCAAAAACCC | GAAGTGTCATCAGCAGCC |
| Â | MPN489 | GATGGTAGTTACCCCGCT | ACTAAAGCGGCAGATCCT |
4 | MPN456 | AGCTGCACAAAGAAGCAC | CTTGAGTGCCGTTACCAC |
5 | MPN011 | AAAGGCATTAGCGATGTTTCA | AATGTTTGTCACCTTTGTGGA |
| Â | MPN012 | AGAGTGCGGAAAAAGGTGAA | AAAGGATCATTGCCTGTTGC |
| Â | MPN411 | GGTATTGCGGAACTTGCTTT | TCAACTTTCCGCTCCATTTT |
| Â | MPN271 | TGCGGATTTTGATTTTGACC | GATCAACCTTTCGCTCCATC |
| Â | MPN505 | TTTGAAAAGGGCGAATTAGG | TGATCAACCTTTCGCTCAAA |
6 | MPN647 | ATGGATCCTTCCCGTTTTTC | CCGGGATAAGTTTCTGCAAG |
| Â | MPN646 | TGAACTGGGCGATAAGGAAG | AACAAATTTGAAGCAGGTGGA |
| Â | MPN645 | TGGCGGAGTAAAAGAAACTGA | TCAAAAGAAGTGGCACCAAA |
| Â | MPN644 | GGAGTGCAAAGCCAAAAAGT | CTTCACCACTGCCAACAATG |
| Â | MPN643 | TGGCAGAAGCATTGAAGATG | TGTGTGTATCTTTCCACCAAGC |
| Â | MPN642 | CCACATAAAGATGGAAGGGATG | TCCAGTATATTCACTTTCTTCTACGC |
| Â | MPN641 | TCAATTGAGGGAAGGCGTTA | TGCAGTGACTCCACAAAAGC |
| Â | MPN640 | TTCGGGAGGTAAAGGTAGCA | TGAATTTGGCTTTTTCACCA |
| Â | MPN639 | CGCGAAAGTTACGGCTTAAA | AAGCGGCCTACTTCAGTTTG |