Skip to main content

Table 2 Effect of robAYp overexpression on the transcript levels of efflux pump genes in Y. pestis

From: A multicopy suppressor screening approach as a means to identify antibiotic resistance determinant candidates in Yersinia pestis

Gene namea

University of Wisconsinb

TIGR

SANGER

Pump family or protein functionc

FCd

(floR)

y2173

NT02YP2579

YPO2148

MFS

12.7 ± 2.5

(yieO)

y0010

NT02YP0010

YPO0009

MFS

8.6 ± 1.1

(acrA*-acrB)

y3392*-y3393

NT02YP4041

YPO1000

RND

6.9 ± 0.4

acrA*-acrB

y1050*-y1049

NT02YP1227

YPO3132

RND

5.0 ± 0.4

(acrA*-acrB)

y3760*-y3759

NT02YP4463

YPO0420

RND

2.7 ± 0.1

-

y4041

NT02YP4789

YPO4020

DMT

1.7 ± 0.9

yegM*-yegN-yegO-yegB

y1386*-y1385-y1384-y1383

NT02YP1646

YPO2847

RND

1.4 ± 0.5

(ybjY*-ybjZ)

y2814*-y2813

NT02YP3366

YPO1364

ABC

1.4 ± 0.4

(acrA*-acrB)

y0702*-y0703

NT02YP0804

YPO3483

RND

1.3 ± 0.3

emrA*-emrB

y0922*-y0921

NT02YP1066

YPO3267

MFS

1.3 ± 0.4

(macA*-macB)

y1481*-y1480

NT02YP1756

YPO2999

RND

1.3 ± 0.5

ygeD

y3180

NT02YP3786

YPO0792

MFS

1.3 ± 0.2

fieF

y0060

NT02YP0067

YPO0077

CDF

1.2 ± 0.1

(abgT)

y3402

NT02YP4052

YPO1008

IT

1.1 ± 0.2

acrD

y1439

NT02YP1705

YPO3043

RND

1.1 ± 0.1

sugE

y0613

NT02YP0702

YPO0355

SMR

1.1 ± 0.4

(ynfM)

y2108

NT02YP2497

YPO2266

MFS

1.1 ± 0.4

bcr

y2916

NT02YP3488

YPO1267

MFS

1.1 ± 0.3

aaeA*-aaeB

y0178*-y0177

NT02YP0192

YPO3685

ArAE

1.1 ± 0.0

(yjcR*-Q)

y3558*-y3559

NT02YP4231

YPO0619

RND

1.0 ± 0.2

yajR

y1017

NT02YP1187

YPO3169

MFS

1.0 ± 0.1

arsB

y0844

NT02YP0968

YPO3347

IT

1.0 ± 0.4

corC

y1191

NT02YP1397

YPO2617

HCC

0.9 ± 0.1

mdtJ*-mdtI

y2242*-y2241

NT02YP2670

YPO2068

SMR

0.8 ± 0.2

rosA

y1087

NT02YP1268

YPO3093

MFS

0.8 ± 0.1

ydhC

y1948

NT02YP2306

YPO2389

MFS

0.8 ± 0.2

 

y3186

NT02YP3792

YPO0798

MFS

0.7 ± 0.1

(ydhE)

y1945

NT02YP2302

YPO2392

MATE

0.7 ± 0.1

(ybeQ)

y1874

NT02YP2220

YPO1712

MFS

0.7 ± 0.0

mdlA*-mdlB

y1039*-y1040

NT02YP1213

YPO3145

ABC

0.7 ± 0.0

ydeF

y2653

NT02YP3168

YPO1515

MFS

0.5 ± 0.1

emrE, gacE

y2000

NT02YP2368

YPO2333

SMR

0.5 ± 0.0

mdfA, cmr

y4067

NT02YP4824

YPO4048

MFS

0.2 ± 0.0

(ydjV)

y2272

NT02YP2703

YPO2040

MFS

nd

  1. a Gene name as annotated for Y. pestis strain KIM and/or CO92 or gene names (in parentheses) given herein based on the name of their E. coli homologs. Multiple names for the same gene are separated by commas. Genes of multi-component pumps are separated by dashes. The star (*) marks genes from multi-component pump gene clusters whose transcripts were analyzed by qRT-PCR. b Gene designations in the University of Wisconsin, TIGR, and SANGER Y. pestis genome databases. The Wisconsin column shows designations for all the genes in each predicted multi-component pump. TIGR and SANGER columns show only genes targeted in qRT-PCR. c Pump families assigned based on homology to known (super)family members from other organisms. MFS, major facilitator superfamily; SMR, small multidrug resistance family; ABC, ATP-binding cassette superfamily; RND, resistance-nodulation-cell division superfamily; MATE, multidrug and toxic compound extrusion family; DMT, drug/metabolite transporter superfamily; CDF, cation diffusion facilitator family; ArAE, aromatic acid exporter family; IT, Ion transporter superfamily; HCC, HlyC/CorC family. d Fold change values (FC) are means of triplicates ± standard errors and are presented in decreasing order. For polycistronic transcripts, qRT-PCR was conducted with primers targeting the first pump component-encoding gene of the operon. These genes are marked with a star. nd, transcript not detected in any of the three Y. pestis strains examined (wild-type, Yp pGEM-4Z, and Yp pGEM-RobYp).
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us