Identification of human pathogens isolated from blood using microarray hybridisation and signal pattern recognition

Table 3 Influence of mismatch type on weighted mismatch calculation

Probe	Target		Probe	Target		Probe	Target		Probe	Target
A -	A	1.0	G -	A	1.0	C -	A	0.7	T -	C	1.0
-	C	0.4	-	G	1.0	-	C	1.0	-	G	1.0
-	G	1.2	-	T	1.0	-	T	1.0	-	T	1.0

Weights of mismatches due to the type of mismatched bases. A mismatch of adenine on the probe with cytosine on the target sequence is weighted with 0.4, whereas a mismatch of the same probe with a guanine in the target sequence is weighted with 1.2.

ISSN: 1471-2180

Probe	Target		Probe	Target		Probe	Target		Probe	Target
A -	A	1.0	G -	A	1.0	C -	A	0.7	T -	C	1.0
-	C	0.4	-	G	1.0	-	C	1.0	-	G	1.0
-	G	1.2	-	T	1.0	-	T	1.0	-	T	1.0

Probe	Target		Probe	Target		Probe	Target		Probe	Target
A -	A	1.0	G -	A	1.0	C -	A	0.7	T -	C	1.0
-	C	0.4	-	G	1.0	-	C	1.0	-	G	1.0
-	G	1.2	-	T	1.0	-	T	1.0	-	T	1.0

Probe	Target		Probe	Target		Probe	Target		Probe	Target
A -	A	1.0	G -	A	1.0	C -	A	0.7	T -	C	1.0
-	C	0.4	-	G	1.0	-	C	1.0	-	G	1.0
-	G	1.2	-	T	1.0	-	T	1.0	-	T	1.0