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Figure 4 | BMC Microbiology

Figure 4

From: Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli

Figure 4

Growth properties of E. coli strains harboring IAA-inducible genes. E. coli strains harboring IAA-inducible genes were grown in LB medium containing 50 μg/ml ampicillin, with or without IAA (100 μg/ml), at 37°C. (A) a; MEC199 (Pw-plsB), b; MEC201 (Pw-plsB ΔplsB), c; MEC212 (Pw-plsB ΔplsB pSTV29 pMW118), d; MEC214 (Pw-plsB ΔplsB pSTV29 pMW118yneS), e; MEC218 (Pw-plsB ΔplsB pSTV29BsplsX pMW118), and f; MEC220 (Pw-plsB ΔplsB pSTV29BsplsX pMW118yneS). (B) a; MEC308 (Pw-EcplsX), b; MEC309 (Pw-ygiH), c; MEC306 (Pw-EcplsX ΔplsX ΔygiH), and d; MEC307 (Pw-ygiH ΔplsX ΔygiH). (C) E. coli strains harboring pUC18S or pUC18S tesA were grown in LB medium containing 50 μg/ml spectinomycin, with or without IAA (50 μg/ml), at 30°C. a; MEC323 (Pw-plsB ΔplsB ΔygiH pUC18S), b; MEC324 (Pw-plsB ΔplsB ΔygiH pUC18S tesA), c; MEC325 (Pw-EcplsX ΔplsX ΔygiH pUC18S), d; MEC326 (Pw-EcplsX ΔplsX ΔygiH pUC18S tesA), e; MEC327 (Pw-ygiH ΔplsX ΔygiH pUC18S), e; MEC328 (Pw-ygiH ΔplsX ΔygiH pUC18S tesA).

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BMC Microbiology

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