Table 2 Strains and plasmids

S. aureus

   CHE482

CC45, ST45, SCCmec_N1 (dfrA, fusB1), blaZ

Mc^r, Tm^r, Fa^r, Sx^R

IMM collection, University Zurich

   ME21

CHE482ΔSCCmec_N1, blaZ

Mc^s, Tm^s, Fa^s, Sx^R

this study

   CHE482Δ

CHE482Δ'SCCmec_N1 (fusB1), blaZ

Mc^s, Tm^s, Fa^r, Sx^R

this study

   ZH81

SCCmec_N1 (dfrA, fusB1), blaZ

Mc^r, Tm^r, Fa^r

[9]

   ME141

ZH81ΔSCCmec_N1, blaZ

Mc^s, Tm^s, Fa^s

this study

   ZH4

SCCmec_N1(dfrA, fusB1), blaZ

Mc^r, Tm^r, Cp^r, Fa^r, Sx^R

[9]

   ME135

ZH4ΔSCCmec_N1, blaZ

Mc^s, Tm^s, Cp^r, Fa^s, Sx^R

this study

   ZH43

SCCmec_N1 (dfrA, fusB1), blaZ, fusA

Mc^r, Tm^r, Cp^r, Fa^r

[9]

   ME138

ZH43ΔSCCmec_N1, blaZ, fusA

Mc^s, Tm^s, Cp^r Fa^r

this study

   HDE288

Pediatric clone, type 4 ccr complex

Mc^r

[24, 43]

E. coli

   DH5α

restriction-negative strain for cloning

Invitrogen

Plasmids

   pYT3

ori(ts), S. aureus, tetL

Tc^r

[35]

   pSR3-1

ori(ts) S. aureus, ccrAB2 genes, tetL

Tc^r

[35]

   pAW17

S. aureus-E. coli shuttle vector, aac-aph

Km^r

[44]

   pME15

pAW17 ccrAB4-1_CHE482

Km^r

This study

   pME21

pYT3 ccrAB4-1_CHE482

Tc^r

This study

   pME22

pYT3 ccrAB4-2_CHE482

Tc^r

This study