From: Purification and preliminary crystallization of alanine racemase from Streptococcus pneumoniae
 | Volume [ml] | Protein Conc [mg/ml] | Total Protein [mg] | Specific Activity [u/mg] | Total Activity [u] |
---|---|---|---|---|---|
Crude Extract | 90.00 | 12.50 | 1125.00 | 5.10 | 5736.54 |
Supernatant after 20 % AmSO4 precipitation | 95.00 | 8.90 | 845.50 | 6.33 | 5358.91 |
Resuspended pellet after 60 % AmSO4 precipitation | 35.00 | 22.50 | 787.50 | 6.74 | 5311.40 |
Before Q-Sepharose | 45.00 | 13.00 | 585.00 | 8.16 | 5143.09 |
After Q-Sepharose | 24.00 | 3.80 | 91.20 | 45.87 | 4174.49 |
After HIC | 10.00 | 7.00 | 70.00 | 49.91 | 3556.27 |
Before Superdex | 8.00 | 3.30 | 26.40 | 64.09 | 1692.09 |
After Superdex | 0.50 | 50.00 | 25.00 | 65.81 | 1645.23 |