Skip to main content

Table 2 Regulatory dependence of type III secreted effectors under SPI-2 inducing conditions.

From: Systematic analysis of the regulation of type three secreted effectors in Salmonella enterica serovar Typhimurium

  

A

B

C

D

E

F

G

H

I

  

wild type

MM + pWSK29

MM sprB+

MM hilC+

MM hilD+

MM hilA +

MM invF+sicA +

MM ssrB+

MM sirA +

1

sopA-lacZ

1221 ± 35

35 ± 13

24 ± 7

19 ± 7

26 ± 7

48 ± 17

6129 ± 1152

75 ± 36

33 ± 6

2

sopB-lacZ

3573 ± 34

99 ± 5

61 ± 7

61 ± 15

78 ± 10

44 ± 3

219791 ± 11811

33 ± 15

46 ± 5

3

sopE2-lacZ

3530 ± 270

5406 ± 547

4807 ± 538

7285 ± 1182

2347 ± 35

7141 ± 852

49681 ± 5581

7141 ± 2059

5222 ± 350

4

slrP-lacZ

1650 ± 24

1222 ± 92

3091 ± 43

1108 ± 132

1211 ± 163

1688 ± 523

1858 ± 238

1901 ± 311

2146 ± 34

5

sspH1-lacZ

3744 ± 140

1908 ± 254

1772 ± 471

1686 ± 161

1420 ± 116

1950 ± 128

3558 ± 232

3947 ± 978

1951 ± 154

6

sspH2-lacZ

6482 ± 1779

764 ± 123

1006 ± 48

655 ± 80

625 ± 84

981 ± 35

850 ± 102

62305 ± 14432

890 ± 111

7

sifA-lacZ

9995 ± 1326

13634 ± 1478

11691 ± 1186

10521 ± 1782

12603 ± 1877

13028 ± 2017

11010 ± 1742

8625 ± 784

21071 ± 1272

8

sifB-lacZ

2209 ± 503

1085 ± 77

711 ± 89

49 ± 3

1264 ± 139

1074 ± 207

43 ± 6

14677 ± 1092

43 ± 8

9

sseI-lacZ

3543 ± 314

119 ± 23

168 ± 40

130 ± 19

127 ± 32

128 ± 25

108 ± 16

11049 ± 2830

165 ± 10

10

sseG-lacZ

3017 ± 780

1444 ± 93

700 ± 49

1503 ± 109

1146 ± 118

690 ± 121

1083 ± 96

4229 ± 221

802 ± 99

  1. The lac fusion being measured is listed in the left column. The strain background is listed along the top. Each strain harbors a lac fusion to an effector and a plasmid encoding a regulator or the vector control. The values in bold are those showing greater than 3-fold differences compared to the MM + pWSK29 strain that are discussed in the text. Each value represents the mean of at least three experiments ± standard deviation. MM, multi-regulator mutant [Δ (avrA-invH)1 ssrB::cat sirA4::hyg].
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us