Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment

BMC Microbiology

Table 3 E. coli MG1655 LSMMG regulated genes in rich (LB) medium.

LSMMG up-regulated genes
Gene	b#	Contran	Ave. Fold Change	Average p-value	Gene Product	Product Location	Present in S . Typhimurium
accA	b0185		2.05	0.2873	acetyl-coenzyme A carboxylase	cytoplasm	present
LSMMG down-regulated genes
apt	b0469		-2.22	0.0005	adenine phosphoribosyltransferase, adenine salvage	cytoplasm	present
sucD	b0729		-2.15	0.0001	succinyl-coA synthetase alpha subunit	cytoplasm	present
rplY	b2185		-7.57	0.0506	50S ribosomal subunit protein L25	cytoplasm	present
rplS	b2606	yes	-2.99	0.0597	50S ribosomal subunit protein L19	cytoplasm	present
trmD	b2607	yes	-2.59	0.0211	tRNA (guanine-1) methyltransferase	cytoplasm	present
rimM	b2608	yes	-2.67	0.0024	protein required for wild-type 16S rRNA processing	cytoplasm	present
rpsP	b2609	yes	-3.38	0.0046	30S ribosomal subunit protein S16	cytoplasm	present
ypjD	b2611		-2.26	0.0011	ORF, putative membrane protein	membrane orthologue
rplU	b3186		-2.41	0.0006	50S ribosomal subunit protein L21	cytoplasm	present
rplA	b3984	yes	-5.33	0.0538	50S ribosomal protein I1	cytoplasm	present
rplJ	b3985	yes	-6.05	0.0542	50S ribosomal subunit protein L10	cytoplasm	present
rpsF	b4200	yes	-3.09	0.0003	30S ribosomal subunit protein S6	cytoplasm	present
rpsR	b4202	yes	-3.31	0.0174	30S ribosomal subunit protein S18	cytoplasm	present
rplI	b4203	yes	-2.81	0.0123	50S ribosomal subunit protein L9	cytoplasm	present

Ave. Fold Change column average of 3 experiments and Average p-value column average p-values from the 2 rich LB media functional genomic experiments.
Underlined: associated or involved in membrane function, production or regulation.

ISSN: 1471-2180