From: Genetic characterization of psp encoding the DING protein in Pseudomonas fluorescens SBW25
Strain, plasmid or primer | Relevant properties | Source/reference |
---|---|---|
P. fluorescens | ||
SBW25 | Wild type strain isolated from sugar beet | [10] |
SBW25-Sm | Spontaneous Smr derivative of SBW25 | [20] |
SBW25ΔdapB | DAP/lysine auxotroph of SBW25 | [20] |
PIL082 | The hxcR-'dapB'lacZ IVET fusion strain of SBW25ΔdapB, Tcr | This work |
PIL082-1 | ΔdapB DUP(hxcS-psp)::pIVETD, the psp-'dapB'lacZ IVET fusion strain, Tcr | This work |
SBW25-lacZ | SBW25 marked with 'lacZ in a phage locus | [28] |
PBR826 | Δpsp or Δpflu2427, SBW25 with a nonpolar deletion of psp, Tcs | This work |
PBR827 | ΔpstC or Δpflu3317, SBW25 with a nonpolar deletion of pstC, Tcs | This work |
PBR828 | ΔhxcR or Δpflu2424, SBW25 with a nonpolar deletion of hxcR, Tcs | This work |
PBR829 | DUP(hxcS-psp)::pUIC3, the psp-'lacZ fusion strain of SBW25, Tcr | This work |
PBR830 | DUP(pstS-pstC)::pUIC3, the pstC-'lacZ fusion strain of SBW25, Tcr | This work |
Plasmid | ||
pRK2013 | Helper plasmid, Tra+, Kmr | [34] |
pUIC3 | Integration vector with promoterless 'lacZ, ori R6K, Mob+, Tcr | [21] |
pIVETD | DAP-based IVET vector, pUIC3 with promoterless 'dapB'lacZ, ori R6K, Tcr | [20] |
pIVETD-13 | pIVETD containing 0.8 kb psp fragment fused to 'dapB'lacZ, ori R6K, Tcr | This work |
pUIC3-61 | pUIC3 carrying psp-'lacZ fusion, ori R6K, Tcr | This work |
pUIC3-62 | pUIC3 carrying pstC-'lacZ fusion, ori R6K, Tcr | This work |
pUIC3-41 | pUIC3 containing 1.6 kb psp deletion fragment, ori R6K, Tcr | This work |
pUIC3-63 | pUIC3 containing 1.6 kb pstC deletion fragment, ori R6K, Tcr | This work |
pUIC3-64 | pUIC3 containing 1.9 kb hxcR deletion fragment, ori R6K, Tcr | This work |
Primera | ||
psp-1 | GAAGATCT GTTGCGTGCCCTGGAAG | |
psp-2 | cagcatgcggatcc gttgacggaAGCGAGGGTCATGGATACCG | |
psp-3 | tccgtcaacggatcc gcatgctgCGGCAACACCAACGTCTGC | |
psp-4 | GAAGATCT GCTGCTGGAAATCCACGGT | |
hxcR-1 | GAGATCT GCGGTAATGGCCACCTATG | |
hxcR-2 | cagcatgcggatcc gttgacggaCGCTGCACCTCGCTGATCGA | |
hxcR-3 | tccgtcaacggatcc gcatgctgTCGACGACGATGTGCGCAGC | |
hxcR-4 | GAGATCT ATGCCAATTCAAACGCGCCA | |
pstC-1 | GAGATCT TCACCAAGCACCTGGCGG | |
pstC-2 | cagcatgcggatcc gttgacggaAGCCGTGCTTTTCCATGCCT | |
pstC-3 | tccgtcaacggatcc gcatgctgTCGCTGTTTGCACCGGCCAAC | |
pstC-4 | GAGATCT GCCGGTGGGCAAGACGATTTTC |