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Table 1 Transformation efficiency of mutant cells compared to wild type cells

From: DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells

Strain

Chromosomal DNA

E. coli plasmid (integrative)

Plasmid (self replicating)

Wild type

100

100

100

ST21 (Smf-YFP)

79.5 (± 4.5)

102 (± 5)

96.8 (± 3.5)

Δsmf (smf::Ery)

10.8 (± 2.0)

0.58 (± 0.25)

1.69 (± 0.25)

ΔrecA (recA::Cm)

0.11 (± 0.05)

0.49 (± 0.05)

49.5 (± 2.5)

ΔrecA, Δsmf

0.08 (± 0.04)

0.24 (± 0.06)

0.94 (± 0.45)

  1. Transformation efficiency was determined by counting number of transformants versus number of viable cells (in triplicate). Numbers are percent relative to wild type (PY79) cells.