Figure 7
From: Acceleration of epithelial cell syndecan-1 shedding by anthrax hemolytic virulence factors
Shedding of Synd1 into culture supernatants (A) and changes in barrier permeability (B) for HSAECs treated with the hemolytic proteins and LT. The cells initially confluent on a 12 mm insert (Costar, 3.0 μm pores) were treated with 1 μg/ml of each protein for 4 h in 1% FCS media. After treatment the change in barrier permeability was tested as increase in the absorbance of Blue Dextran 2000 added for 2 h to the culture media in the top chamber and detected in the lower chamber, in comparison with untreated cells. Error bars represent 95% confidence intervals; 3 culture plate wells were used for each measurement.