Figure 3

Key carbon sources that were not metabolized by the E. coli O157:H7 hns mutant. Some of the metabolic intermediates and substrates that were not (□, open box) metabolized as sole carbon sources by the hns mutant of E. coli O157:H7 when examined using Phenotypic Microarrays. Both the parent and hns mutant strains metabolized the compounds that are not in boxes. Compounds in italics which were not tested (a). E. coli converts glutamate and oxaloacetate to α-ketoglutarate and aspartate (b) [55,56]. Glycyl-L-proline was tested (c). α-ketoglutarate was not metabolized as a sole carbon source by neither the parent strain nor the hns mutant (d).