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Table 4 Comparison of microscopical and PCR-based determination of Coxiella cell numbers. The PCR measurements were performed in duplicate; mean values are shown.

From: Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii

Coxiella particles per μl

Determined microscopicallya

Quantified by PCRb

4.2 × 106

5.00 × 106

4.2 × 105

3.05 × 105

4.2 × 104

3.15 × 104

4.2 × 103

2.45 × 103

4.2 × 102

1.45 × 102

4.2 × 101

1.70 × 101

4.2 × 100

0

  1. aCell numbers were only counted from undiluted sample.
  2. bReal-time PCR targeting the icd marker.