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Table 3 PCR quantification of DNA dilutions of the C. burnetii Nine Mile RSA493 strain. The measurements were performed in duplicate; mean values are shown.

From: Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii

 

Calculated Valuesa

Measured Valuesb

 

DNA conc [pg/μl]

icd

IS1111

icd

IS1111

IS per genomec

2900

1.3 × 106

2.6 × 107

4.5 × 106

5.9 × 107

13.1

290

1.3 × 105

2.6 × 106

5.2 × 105

8.1 × 106

15.6

29

1.3 × 104

2.6 × 105

3.9 × 104

5.0 × 105

12.8

2.9

1.3 × 103

2.6 × 104

2.8 × 103

3.5 × 104

12.5

0.29

1.3 × 102

2.6 × 103

2.3 × 102

2.6 × 103

11.3

2.9 × 10-2

1.3 × 101

2.6 × 102

1.5 × 101

2.6 × 102

17.3

  1. aNumber of target copies based on DNA concentration and genome length.
  2. bNumber of target copies based on PCR quantification using plasmid standards.
  3. cCalculated as IS1111 measured per icd measured.
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BMC Microbiology

ISSN: 1471-2180

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