Figure 2
Sensitivity of detection of the real-time PCR using pure culture of E. sakazakii. (A, B) Representative amplification plots obtained with 450 IAC molecules and decreasing concentration of cells of E. sakazakii ATCC 29544 (A) and ATCC 51329 (B): 3.6 × 107 (1), 3.6 × 106 (2), 3.6 × 105 (3), 3.6 × 104 (4), 3.6 × 103 (5), 3.6 × 102 (6) and 36 CFU/ml (7), corresponding to 1.8 × 106 (1), 1.8 × 105 (2), 1.8 × 104 (3), 1.8 × 103 (4), 1.8 × 102 (5), 18 (6) and 1.8 (7) cells per tube. C-: negative control. (C, D) Standard curves generated by plotting the log of cell numbers per tube of strain ATCC 29544 (C) or ATCC 51329 (D) versus the number of cycles required to reach the C T values.