Figure 7
From: Gel shift analysis of the empA promoter region in Vibrio anguillarum
Gel shift analysis of the V. anguillarum vanT mutant, NB02 in NSSM and LB20. The same labeled oligomer used in Figures 1 and 2 was added to protein extracts from NB02 cells incubated in NSSM and LB20. DIG-labeled DNA alone was added to the lane marked with an asterisk (*). Lane 1, 5 μg of protein extract from cells at time 0 h; lane 2, 5 μg protein extract from cells at time 3 h; lane 3, 5 μg protein extract from cells at time 3 h plus unlabeled lux box-empA promoter; and lane 4, 5 μg protein extract from cells at time 3 h plus the oct2A (from E. coli) unlabeled oligomer. The data presented are representative of three replicate experiments.