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Table 2 Colonies (CFU per ml) recovered from temperature sensitivity tests of pB264 derivative.

From: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus

 

Parent I24(pAL298) culture grown in LB at:

plated onto 1

30°C

37°C

LB

1.8 ± 0.2 × 108

2.2 ± 0.7 × 108

LB + gentamicin

1.1 ± 0.1 × 108

1.3 ± 0.6 × 105

  1. 1composition of the medium used to test aliquots of serial dilutions; these plates were all incubated at 30°C