Table 1 Bacterial strains used in this study

B. subtilis

168

trpC2 (wild type)

BGSC^a, 1A1

MD153

trpC2 ptsG::cat

[40]

MD186

trpC2 glcK::spc

[9]

MD189

trpC2 glcK::spc ptsGHI::erm

[9]

MD191

trpC2 glcK::spc ptsG::cat

[9]

MD195

trpC2 amyE::(cat-lacZ)

pAC6 tf> 168^b

MD196

trpC2 amyE::(2112 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P01 tf> 168^b

MD197

trpC2 amyE::(1994 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P011 tf> 168^b

MD198

trpC2 amyE::(1673 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P012 tf> 168^b

MD199

trpC2 amyE::(974 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P013 tf> 168^b

MD202

trpC2 amyE::(659 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P014 tf> 168^b

MD204

trpC2 amyE::(464 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P021 tf> 168^b

MD205

trpC2 amyE::(1163 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P031 tf> 168^b

MD206

trpC2 amyE::(1046 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P032 tf> 168^b

MD207

trpC2 amyE::(1308 bp Eco RI-Bam HI fragment-lacZ, cat)

pLR-P015 tf> 168^b

MD229

trpC2 gluP::aphA3

pLM-YqgP-Km1 tf> 168^b

MD230

trpC2 glcK::spc gluP::aphA3

pLM-YqgP-Km1 tf> MD186^b

MD231

trpC2 glcK::spc ptsGHI::erm gluP::aphA3

pLM-YqgP-Km1 tf> MD189^b

MD232

trpC2 ptsG::cat gluP::aphA3

pLM-YqgP-Km1 tf> MD153^b

MD233

trpC2 glcK::spc ptsG::cat gluP::aphA3

pLM-YqgP-Km1 tf> MD191^b

MD234

trpC2 ptsGHI::erm gluP::aphA3

Chr. DNA QB6020 tf> MD229^b

QB6020

trpC2 ptsGHI::erm

[51]

E. coli

TG1

supE hsd△ 5 thi△ (lac-proAB) F'(traD36 proAB⁺ lacI^q lacZ△ M15

[52]