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Figure 1 | BMC Microbiology

Figure 1

From: Staphylococcus aureus - induced tumor necrosis factor - related apoptosis - inducing ligand expression mediates apoptosis and caspase-8 activation in infected osteoblasts

Figure 1

Western immunoblot analysis of TRAIL expression from normal human osteoblasts 24 h following incubation with LPS, peptidoglycan, teichoic acid (TA, 1 μg), staphylococcal enterotoxin A (SEA, 1 μg), or following infection with S. aureus at a MOI of 250:1 (S.a. 250). S. aureus infection was for 45 min, followed by washing and gentamicin treatment of osteoblasts for 24 h prior to protein isolation. Ten micrograms of total osteoblast protein were loaded for each lane.

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