Bacterial Strain or Plasmid | Relevant Characteristics | Source or Reference |
---|---|---|
E. coli | Â | Â |
DH5α | EndA1, hsdR17, supE44, thi-1, recA1, gyrA96, relA1, • (argF-lacZYA), U169, φ 80dlacZ ΔM15 | Life Technologies |
S17-1 | Spr. RP4 tra region, mobilizer strain | [51] |
Rhizobium leguminosarum | ||
VF39SM | biovar viciae, Smr | [52] |
HLG1 | VF39SM, mcpG:: ΩSp; Smr, Spr | This work |
HLG2 | VF39SM, mcpG:: ΩSp; Smr, Spr | This work |
LRS39401 | VF39SM cured of pRleVF39d | [48] |
LRS39601 | VF39SM cured of pRleVF39f | [48] |
T3CB | biovar viciae, 1062 derivative, | [53] |
W14-2 | biovar trifolii, Smr | [18] |
8002 | biovar phaseoli | [54] |
8401 | 8002 cured of pSym plasmid | [54] |
GF160 | biovar viciae, Smr, | [55] |
3841 | biovar viciae, JB300 derivative | [56] |
162Y10 | biovar trifolii | [57] |
Plasmids | ||
pBSIISK(+) | Cloning vector, Apr | Stratagene |
pCYΩSp | Sp resistance gene from pHP45Ω [58] flanked by MCS of p1918 | [35] |
p1918 | vector with a MCS composed of inverted repeats of the pUC19 MCS, Apr | [59] |
pJQ200mp18 | delivery vector for gene replacement, Gmr | [34] |
pKC42 | 6 kb Bam HI VF39SM genomic fragment containing mcpG cloned into pBSIISK(+) | This work |
pCYG1 | Bcl I fragment with mcpG ORF cloned into pJQ200mp18 | This work |
pCYG2 | Derivative of pCYG1 with ΩSp cassette cloned into mcpG gene | This work |