Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan

Table 2 Bacteriological characterization, virulence gene profile and cdt subtype of CDT-producing Escherichia coli isolated from cattle and swine in Japan

Host	CDT subtype	Serotype	PG¹	n=	CDT-III and -V subtyping				Virulence gene
									DEC⁸					Adhesin⁹				NTEC¹⁰
					*cdt-IIIi* ²	*cdt-Vi* ³	*cdt-IIIABC* ⁴	*cdt-V* up⁵/down⁶	*stx1*	*stx2*	*subAB*	*eaeA*	*astA*	*saa*	*lpfAO113*	*ehaA*	*iha*	*cnf2*
Cattle	CDT-I	O112ac:H20	B1	1	ND⁷	ND	ND	ND/ND	-	-	-	-	-	-	+	+	-	-
		OUT:H26	D	1	ND	ND	ND	ND/ND	-	-	-	-	-	-	-	+	-	-
	CDT-IV	O169:H10	B2	1	ND	ND	ND	ND/ND	-	-	-	-	+	-	-	-	-	-
	CDT-III	O2:HUT	B2	3	+	-	+	-/-	-	-	-	-	-	-	-	-	-	+
		O2:NM	B2	1	+	-	+	-/-	-	-	-	-	-	-	-	-	-	+
		O7:H6	B1	1	+	-	+	-/-	-	-	-	-	-	-	+	+	-	+
		O88:H2	B1	1	+	-	+	-/-	-	-	-	-	-	-	+	+	-	+
		O88:H4	B1	1	+	-	+	-/-	-	-	-	-	+	-	+	+	-	+
		O88:H6	B1	1	+	-	+	-/-	-	-	-	-	-	-	+	+	-	+
		OUT:H1	B1	1	+	-	+	-/-	-	-	-	-	-	-	+	+	-	+
		OUT:H21	B1	11	+	-	+	-/-	-	-	-	-	+	-	+	+	-	+
		OUT:H45	D	1	+	-	+	-/-	-	-	-	-	-	-	-	+	-	+
		OUT:HUT	B1	1	+	-	+	-/-	-	-	-	-	-	-	+	+	-	+
		OUT:NM	B1	3	+	-	+	-/-	-	-	-	-	-	-	+	+	-	+
	CDT-V	O2:H10	B2	1	-	+	-	+/+	-	-	-	-	-	-	-	-	-	-
		O8:HUT	B1	1	-	+	-	+/+	-	-	-	-	-	-	+	-	-	+
		O22:H8	B1	5	-	+	-	+/+	+ (4¹²/5¹³)	+ (4/5)	-	-	+ (1/5)	+	+	+	+	-
		O22:HUT	B1	2	-	+	-	+/+	+	+	-	-	-	+	+	+	+	-
		O113:H21	B1	3	-	+	-	+/+	-	+	+	-	-	+	+	+	+	-
		O113:NM	B1	2	-	+	-	+/+	-	+	+	-	-	+	+	+	+	-
		O118:NM	B1	1	-	+	-	+/+	-	-	-	-	+	-	+	+	-	-
		O154:H34	B1	1	-	+	-	+/+	-	-	-	-	-	-	+	+	+	-
		O156:HUT	B1	3	-	+	-	+/+	-	-	-	+¹¹	-	-	+	+	-	-
		O163:HUT	B1	1	-	+	-	+/+	-	-	-	-	+	-	+	+	-	-
		OUT:H1	B1	1	-	+	-	+/+	-	-	-	-	-	-	+	-	-	+
		OUT:H19	B1	2	-	+	-	+/+	-	-	-	-	+	-	+	+	-	-
		OUT:H2	B1	5	-	+	-	+/+	-	-	-	-	-	-	+	-	-	+
		OUT:H21	B1	1	-	+	-	+/+	-	-	-	-	-	-	+	+	-	-
		OUT:H25	B1	1	-	+	-	+/+	+	+	+	-	-	+	+	+	+	-
		OUT:H48*	D	2	-	+	-	-/-	-	-	-	-	+	-	-	+	-	-
		OUT:H6	B1	2	-	+	-	+/+	+	+	-	-	-	+	+	+	+	-
		OUT:H8	B1	5	-	+	-	+/+	+	+	-	-	-	+	+	+	+	-
		OUT:HUT	B1	7	-	+	-	+/+	+ (2/7)	+ (2/7)	-	-	+ (4/7)	+ (2/7)	+	+	+ (2/7)	-
		OUT:NM	B1	6	-	+	-	+/+	-	+ (1/6)	+ (1/6)	-	+ (5/6)	+ (1/6)	+	+	+ (1/6)	-
	CDT-III and V	O2:HUT*	B2	1	+	+	-	-/-	-	-	-	-	+	-	-	-	-	-
Swine	CDT-V	O98:H10*	B1	5	-	+	-	-/+	-	-	-	-	+	-	+	+	-	-
		OUT:HUT*	B1	1	-	+	-	-/+	-	-	-	-	+	-	+	+	-	-
	CDT-II	O84:NM¹⁴	D	1	ND	ND	ND	ND/ND	-	-	-	+	-	-	-	-	-	-

bfp, EAF, elt, est, aggR, invE genes for DEC, cnf1 for NTEC, and efa1 for adhesin were negative in all strains tested.
*Not properly differentiated by previous type-specific PCR assays, ¹phylogenetic group, ²PCR result by CdtIII/VB-F and CdtIIIC-R primers, ³PCR result by CdtIII/VB-F and CdtVC-R primers, ⁴PCR result by Cdt-IIIAf and Cdt-IIIACr primers ⁵PCR result by P2-A2 and cdtA-F primers, ⁶PCR result by cdtC-F and P2-C3 primers, ⁷not done, ⁸genes for DEC, ⁹genes for Adhesin, ¹⁰gene for NTEC, ¹¹eae-θ/γ2, ¹²No. of positive strains, ¹³No. of tested strains, ¹⁴identified as Escherichia albertii.

ISSN: 1471-2180